Dot Drug Testing

DOT Drug Testing - Compliant, Certified & Mobile

The U.S. Department of Transportation (DOT) requires drug and alcohol testing for all employees in safety-sensitive transportation roles. These regulations are governed by 49 CFR Part 40, which defines the procedures for collection, laboratory testing, and Medical Review Officer (MRO) verification.

DOT-regulated industries include:

FMCSA - Federal Motor Carrier Safety Administration (commercial trucking, CDL drivers)
FTA - Federal Transit Administration (public transit)
FAA - Federal Aviation Administration (aviation safety-sensitive employees)
FRA - Federal Railroad Administration (railroad employees)
PHMSA - Pipeline and Hazardous Materials Safety Administration
USCG - U.S. Coast Guard (maritime safety-sensitive workers)

Non-compliance with DOT drug testing requirements can result in significant fines, loss of operating authority, and liability exposure. Priority Diagnostics Inc ensures your program meets every federal requirement.

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DOT Testing Circumstances We Cover

Pre-Employment - Required before a new hire enters a safety-sensitive role.
Random Testing - Unannounced, DOT-mandated random selection from a compliant pool.
Post-Accident - Required after DOT-qualifying accidents meeting specific criteria.
Reasonable Suspicion - When a trained supervisor documents observable impairment.
Return-to-Duty - Following a violation, before the employee resumes safety-sensitive duties.
Follow-Up Testing - Unannounced testing following return-to-duty, per SAP-defined schedule.

DOT Random Testing Consortium

For small employers who cannot maintain a statistically valid random pool independently, Priority Diagnostics Inc offers DOT Random Testing Consortium enrollment. We handle selection management, notification coordination, and on-site mobile collections – keeping you compliant with DOT annual random testing rate requirements.

Other Services

Schedule DOT Testing

Our DOT Collection Process

Every DOT collection performed by Priority Diagnostics Inc follows the strict procedural requirements of 49 CFR Part 40:

Step 1

Donor Verification

Donor identity verified with government-issued photo ID.

Step 2

Chain of Custody

Federal Chain-of-Custody Form (CCF) completed accurately.

Step 3

Specimen Collection

Specimen collected under proper controlled conditions.

Step 4

Validity Testing

Specimen validity testing and split specimen labeling performed.

Step 5

Specimen Transport

Specimen shipped overnight to SAMHSA-certified laboratory.

Step 6

Specimen Review

MRO reviews laboratory result and contacts donor if necessary.

Step 7

Final Results

Final verified result reported to Designated Employer Representative (DER).

Ready to Schedule DOT Testing?

Keep your fleet, crew, and operation compliant. Schedule DOT-compliant mobile testing with Priority Diagnostics today